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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Cancer Cell. 2017 Dec 11;32(6):748–760.e6. doi: 10.1016/j.ccell.2017.11.003

Figure 4. p53 Activation Upregulates Pro-Apoptotic Proteins and Reduces Anti-Apoptotic Mcl-1.

Figure 4

(A) Relative mRNA expression of MDM2, BAX, BAK, and BBC3 (Puma-encoding gene) in OCI-AML3 cells after 12 hr treatment with 1 μM RG.

(B) Immunoblots showing the levels of indicated proteins in OCI-AML3 cells after treatment with 2 μM RG for indicated time. c-PARP-1, cleaved PARP-1 protein.

(C) Immunoblot of Mcl-1 in OCI-AML3 cells treated with indicated concentrations of ABT for 24 hr.

(D) Immunoblots of Mcl-1 in OCI-AML3 cells treated with indicated concentrations of RG for 24 hr (upper left) or treated with 1 μM RG for the indicated durations (lower left) and the MCL1 mRNA levels in OCI-AML3 cells incubated without (Ctrl) or with 1 μM RG for 12 hr.

(E) Changes in Mcl-1 protein levels in OCI-AML3 cells treated with vehicle, 1 μM ABT, 1 μM RG, or the combination for 6, 12, or 24 hr.

Data in bar graphs (A, D) represent the means of triplicate experiments. Error bars, mean ± SD. p values were calculated using two-tailed unpaired Student’s t-test.