REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-p53 (clone DO-1)	Santa Cruz Biotechnology	Cat#sc-126; RRID: AB_628082
Mouse monoclonal anti-MDM2 (clone D-12)	Santa Cruz Biotechnology	Cat#sc-5304; RRID: AB_627918
Mouse monoclonal anti-Bcl-2 (clone C-2)	Santa Cruz Biotechnology	Cat#sc-7382; RRID: AB_626736
Rabbit polyclonal anti-Mcl-1 (clone S-19)	Santa Cruz Biotechnology	Cat#sc-819; RRID: AB_2144105
Rabbit polyclonal anti-Puma (clone H-136)	Santa Cruz Biotechnology	Cat#sc-28226; RRID: AB_2064827
Mouse monoclonal anti-ERK (cloned D-2)	Santa Cruz Biotechnology	Cat#sc-1647; RRID: AB_627547
Mouse monoclonal anti-N-Ras (clone F-155)	Santa Cruz Biotechnology	Cat#sc-31; RRID: AB_628041
Mouse monoclonal anti-GSK3 (clone 0011-A)	Santa Cruz Biotechnology	Cat#sc-7291; RRID: AB_2279451
Rabbit polyclonal anti-PARP-1	Cell Signaling Technology	Cat#9542; RRID: AB_2160739
Rabbit polyclonal anti-Bim	Cell Signaling Technology	Cat#2819; RRID: AB_10692515
Rabbit monoclonal anti-Bak (clone D4E4)	Cell Signaling Technology	Cat#12105
Rabbit monoclonal anti-cleaved caspase-3 (D175) (clone 5A1E)	Cell Signaling Technology	Cat#9664; RRID: AB_2070042
Mouse monoclonal anti-caspase-9 (clone C9)	Cell Signaling Technology	Cat#9508; RRID: AB_10695598
Rabbit monoclonal anti-pMcl-1 (T163) (clone D5M9D)	Cell Signaling Technology	Cat#14765
Rabbit monoclonal anti-B-Raf (clone 55C6)	Cell Signaling Technology	Cat#9433; RRID: AB_ 2259354
Rabbit polyclonal anti-pB-Raf (S445)	Cell Signaling Technology	Cat#2696; RRID: AB_390721
Rabbit polyclonal anti-JNK	Cell Signaling Technology	Cat#9252; RRID: AB_2250373
Mouse monoclonal anti-pJNK (T183Y185) (clone G9)	Cell Signaling Technology	Cat#9255; RRID: AB_2307321
Rabbit polyclonal anti-Ubiquitin	Cell Signaling Technology	Cat#3933; RRID: AB_2180538
Rabbit monoclonal anti-pERK (T202/Y204) (clone D13.14.4E)	Cell Signaling Technology	Cat#4370; RRID: AB_2315112
Rabbit polyclonal anti-pMEK (S217/S221)	Cell Signaling Technology	Cat#9121; RRID: AB_331648
Mouse monoclonal anti-MEK (clone L38C12)	Cell Signaling Technology	Cat#4694; RRID: AB_10695868
Mouse monoclonal anti-AKT (clone 40D4)	Cell Signaling Technology	Cat#2920; RRID: AB_1147620
Rabbit polyclonal anti-pAKT(S473)	Cell Signaling Technology	Cat#9271; RRID: AB_329825
Rabbit monoclonal anti-pGSK3 (S21/S9) (clone D17D2)	Cell Signaling Technology	Cat#8566; RRID: AB_10860069
Mouse monoclonal anti-Bax (clone 2D2)	Sigma-Aldrich	Cat#B8554; RRID: AB_258645
Mouse monoclonal anti-β-Actin (clone AC-74)	Sigma-Aldrich	Cat#A2228; RRID: AB_476697
Mouse monoclonal anti-Noxa (clone 114C307)	Abcam	Cat#ab13654; RRID: AB_300536
Rabbit polyclonal anti-pMcl-1 (S159)	Abcam	Cat#ab111574; RRID: AB_10865697
Mouse monoclonal anti-p21WAF1 (clone EA10)	EMD Millipore	Cat#OP64; RRID: AB_2335868
IRDye® 680RD Donkey anti-Rabbit IgG (H + L)	Li-COR Biosciences	Cat#925-68073
IRDye® 800CW Donkey anti-Mouse IgG (H + L)	Li-COR Biosciences	Cat#925-32212
Mouse monoclonal anti-human CD45 (clone PD7/26 + 2B11)	DAKO	Cat#M0701; RRID: AB_2314143
Mouse monoclonal anti-human CD45-PE (clone HI30)	BD Biosciences	Cat#555483; RRID: AB_395875
Rat monoclonal anti-mouse CD45-APC (clone 30-F11)	BD Biosciences	Cat#559864; RRID: AB_398672
Chemicals, Peptides, and Recombinant Proteins
RG7388 (idasanutlin)	Roche/Genentech	N/A
ABT-199 (venetoclax/GDC-0199)	AbbVie	N/A
A-1210477	AbbVie	N/A
Annexin V, FITC conjugate	BD Biosciences	Cat#556419
Annexin V, APC conjugate	BD Biosciences	Cat#550474
Annexin V, Alexa Fluor 488 conjugate	Thermo Fisher	Cat#A13201
D-Luciferin Firefly Potassium Salt	Gold Biotechnology	Cat#LUCK-1G CAS#115144-35-9
Protease/Phosphatase Inhibitor Cocktail (100X)	Cell signaling	Cat#5872
PD0325901	Selleckchem	Cat#S1036
Trametinib	Selleckchem	Cat#S2673
Binimetinib	Selleckchem	Cat#S7007
CHIR-99021	Selleckchem	Cat#S1263
Bortezomib	Selleckchem	Cat#S1013
Ixazomib	Selleckchem	Cat#S2180
BIO-Acetoxime	Selleckchem	Cat#S7915
MK-2206 2HCl	Selleckchem	Cat#S1078
Critical Commercial Assays
Click-iT EdU Alexa Fluor 647 Imaging Kit	Thermo Fisher	Cat#C10340
FxCycle PI/RNase Staining Solution	Thermo Fisher	Cat#F10797
Q5 Site-Directed Mutagenesis Kit	New England BioLabs	Cat#E0554S
Q5 Hot Start High-Fidelity 2X Master Mix	New England BioLabs	Cat#M0494S
jetPRIME Versatile Transfection Reagent	Polyplus	Cat#114-15
Accell siRNA Delivery Media	Dharmacon	Cat#B-005000-500
QIAprep Spin Miniprep Kit	QIAGEN	Cat#27104
Wizard® SV Gel and PCR Clean-Up System	Promega	Cat#A9281
Experimental Models: Cell Lines
Human: OCI-AML3	DSMZ	Cat#ACC-582; RRID: CVCL_1844
Human: MV-4-11	ATCC	Cat#CRL-9591; RRID: CVCL_0064
Human: MOLM-13	DSMZ	Cat#ACC-554; RRID: CVCL_2119
Human: HL-60	ATCC	Cat#CCL-240; RRID: CVCL_0002
Human: KG1	ATCC	Cat#CCL-246; RRID: CVCL_0374
Human: THP1	ATCC	Cat#TIB-202; RRID: CVCL_0006
Human: HEK293T	ATCC	Cat#CRL-3216; RRID:CVCL_0063
Experimental Models: Organisms/Strains
Mice: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ	The Jackson Laboratory	Strain: 005557; RRID: IMSR_JAX:005557
Oligonucleotides
pLKO.1-TRC shRNA negative control: ccgcaggtatgcacgcgt	Moffat et al., 2006 A gift from David Root	Addgene Plasmid #10879
pMKO.1 puro p53 shRNA 2: gactccagtggtaatctacttcaagagagtagattacactggagtcttttt	Masutomi et al., 2003 A gift from William Hahn	Addgene Plasmid #10672
shRNA targeting human MCL1: ccggtgtgaattcatgggctcatcttcaagagagatgagcccatgaattcacttttttgg	This paper	N/A
TRC lentiviral human CDKN1A shRNA: ccagcactcttaggaacctct (#1)	Dharmacon	Cat#RHS3979-200795837 Clone ID: TRCN0000010401
TRC lentiviral human CDKN1A shRNA: taaggcagaagatgtagagcg (#2)	Dharmacon	Cat#RHS3979-201789662 Clone ID: TRCN0000040123
Accell siRNA non-targeting control pool	Dharmacon	Cat#D-001910-10
Accell MAPK1 siRNA SMART pool	Dharmacon	Cat#E-003555-00
Accell MAPK3 siRNA SMART pool	Dharmacon	Cat#E-003592-00
Accell GSK3B siRNA SMART pool	Dharmacon	Cat#E-003010-00
Accell GSK3A siRNA SMART pool	Dharmacon	Cat#E-003009-00
Recombinant DNA
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro	System Biosciences	Cat#CD550A-1
pLKO.1 Vector	Moffat et al., 2006	Addgene Plasmid #10878
psPAX2 Plasmid	A gift from Didier Trono	Addgene Plasmid #12260
pMD2.G Plasmid	A gift from Didier Trono	Addgene Plasmid #12259
Software and Algorithms
Image Studio Lite Software	LI-COR Biosciences	https://www.licor.com/bio/products/software/image_studio_lite/
Prism Software v6.0	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
CalcuSyn 2.0 Software	Biosoft	http://www.biosoft.com/w/calcusyn.htm
Kaluza Analysis Software 1.3	Beckman Coulter	https://www.beckman.com/coulter-flow-cytometers/software/kaluza
CellSens Software	OLYMPUS	https://www.olympus-lifescience.com/en/software/cellsens/
Other
CountBright™ Absolute Counting Beads	Thermo Fisher Scientific	Cat#C36950
Xenogen IVIS-200 In Vivo Imaging System	PerkinElmer	N/A
Odyssey Infrared Imaging System	LI-COR	N/A
BX43 Upright Microscope with DP72 Digital Color Camera	OLYMPUS	N/A
Vi-CELL XR Viability Analyzer	Beckman Coulter	Mode# 731050
Gallios 10 Colors/3 Lasers Flow Cytometer (561 with Kaluza Acquisition Software)	Beckman Coulter	Mode# B43618