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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Cancer Cell. 2017 Nov 16;32(6):824–839.e8. doi: 10.1016/j.ccell.2017.10.011

Figure 5. NRF2 Mediates Pancreatitis-Accelerated Neoplastic Progression and Induction of Stemness Genes.

Figure 5

(A) Quantification of ADM and PanIN lesions in pancreata of 5-week-old mice of indicated genotypes (n = 9). (B) H&E staining of above tissues. Insets show representative PanIN3 in NRF2-expressing tissue and PanIN1 in NRF2-deficient tissue. Scale bars: 50 µm; inset: 25 µm. (C) Kaplan-Meier survival curves of indicated mouse strains (n = 10). (D) IB analysis of pancreatic lysates from above mice. (E, F) Q-RT-PCR analysis of RNA isolated from acinar cells that were cultured for 3 days in Matrigel after transfection with empty or p62 expression vectors (3 µg; n = 3). KrasG12D (E). KrasG12D ;Nrf2−/− (F). (G) Sphere formation by control and NRF2-ablated MIA PaCa-2 and Capan-2 cells with or without NICD1 overexpression. (H) Quantification of SA-β-gal staining of cells as in G. Results in A, E–H are mean ± SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001. Statistical significance was calculated using Student’s t test (A, E–H) or log-rank test (C). See also Figure S5.