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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Curr Biol. 2017 Nov 22;27(23):3734–3742.e5. doi: 10.1016/j.cub.2017.10.074

Fig 4. Ae. albopictus HP-I Peptides are Potent Activators of Ae. aegypti NPYLR1.

Fig 4

(A) Schematic of normal within-species mating (left and middle) and cross-species satyrization of Ae. aegypti females by Ae. albopictus males (right).

(B) Relative levels of mature Aedes aegypti HP-I and Aedes albopictus HP-I RWamide and RFamide peptides detected by LC-MS in whole adult mosquitoes of the indicated sex and species.

(C) Legend for cell-based assay experiments in C–G, including the amino acid sequences of predicted mature HP-I in Ae. albopictus compared to Ae. aegypti HP-I.

(D) Dose-response curves of Ae. albopictus HP-I peptides (W and F) on Ae. albopictus receptors.

(E) Dose-response curve of Ae. aegypti HP-I on Ae. aegypti NPYLR1.

(F) Dose-response curves of Ae. aegypti HP-I on Ae. albopictus neuropeptide receptors.

(G) Dose-response curves of Ae. albopictus HP-I peptides (W and F) on Ae. aegypti NPYLR1.

(H) Responses to 10µM Ae. aegypti and Ae. albopictus HP-I peptides. Data in C–G are shown as max ratio (maximum fluorescence level/baseline fluorescence level) (mean ± SEM, 3 replicates. *** p < 0.0001, 1-way ANOVA followed by Tukey’s multiple comparisons test).

(I) Schematic of activity of Ae. aegypti and Ae. albopictus HP-I peptides against Ae. aegypti NPYLR1 and Ae. albopictus sNPFR.