Table 1.
Overview of the molecular characterization at different markers of ten DNA isolates (Ha1.1–Ha1.10) from the intestinal mucosa of a white-tailed sea eagle and the Sarcocystis species identified in each isolate. The GenBank accession numbers of sequences obtained from particular isolates are given in parenthesis behind the isolates of origin.
| Species | Cox1a | 18S rRNA gene | ITS1 | 28S rRNA gene |
|---|---|---|---|---|
| S. halieti | Ha1.1 (MF946583)b, Ha1.2, Ha1.5, Ha1.6 | Ha1.6 (MF946587) |
Ha1.6 (MF946589–MF946596) | Ha1.1 (MF946610) |
| S. lari | Ha1.3 (MF946584)c, Ha1.4, Ha1.7, Ha1.8, Ha1.9, Ha1.10 | Ha1.8 (MF946588) | Ha1.8 (MF946597–MF946609) | Ha1.8 (MF946611) |
| S. truncata | Ha1.1 (MF946585)d, Ha1.3, Ha1.6; Ha1.8 (MF946586)d, Ha1.9, Ha1.10 |
(Ha1.1, Ha1.2) (mixed sequences)e |
(Ha1.1, Ha1.2) (mixed sequences)e |
(Ha1.1, Ha1.2) (mixed sequences)e |
DNA of S. truncata was identified with primer pair SF4/SR8D in isolates in which either S. halieti or S. lari were identified with primer pair SF1/SR5.
Identical sequences were obtained from all four isolates of S. halieti.
Identical sequences were obtained from all six isolates of S. lari.
Each sequence type was found in three isolates of S. truncata.
Short fragments of the mixed and largely unintelligible sequences were compatible with sequences of S. truncata.