Extended Data Figure 7. Biasing pre-existing conformational heterogeneity in the LNX2^PDZ2 ground state structure by the external electric field: additional examples.

a, b, A high-resolution (1.1 Å) room-temperature structure of the voltage-OFF ground state of LNX2^PDZ2 (Extended Data Table 3), shows partial occupancy of N415 (a) and D368 (b) in two rotameric states (left). This pre-existing conformational equilibrium is biased in the presence of the electric field (6 kV, 200 ns delay), such that the up and down models each adopt one of the two ground state configurations (middle and right). This supports the result shown in Fig. 4g.