Fig. 3.

Validation of microarray data by qRT-PCR analysis. (A) qRT-PCR analysis. Randomly selected 20 genes were validated by qRT-PCR analyses using the same RNA extracted for microarray analysis. Error bar is SEM from three independent experiments. Significance was assessed using unpaired t-test (*p<0.05, ***p<0.01, ***p<0.001). (B) Spearman's rho. Expression pattern of the 20 genes tested by qRT-PCR is highly correlated to that observed in microarray analysis, with a Spearman's correlation coefficient value of 0.9173 (P<0.0001).