Fig. 5.

Trehalose exerted effects on the Keap1–Nrf2 pathway by altering p62 protein expression. (A-D) Hepa1-6 cells were treated with 50 mM trehalose for 24 h and harvested, while untreated cells served as a control. Expression of Ho-1 (A), Nqo1 (B), MnSOD (C) and Gpx4 (D) mRNA analyzed by real-time RT-PCR (n = 4). Data were normalized to Rps18 expression (n = 4). Values are means ± SD. Differences between values were analyzed by Student's t-test. Statistical significance shown as *p < 0.05, **p < 0.01.