Fig. 6.

Trehalose suppressed oxidative stress. (A, B) Hepa1-6 cells were pretreated with 50 mM trehalose or sucrose, or 10 mM N-acetylcysteine (NAC) for 24 h before incubation with 2 mM paraquat for 15 h. Intracellular ROS levels were analyzed with CM-H₂DCFDA (n = 5). Values indicate means ± SD. Differences among values were analyzed by the Tukey-Kramer method with *p < 0.05, **p < 0.01.