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. Author manuscript; available in PMC: 2017 Dec 14.
Published in final edited form as: Mol Pharm. 2016 Apr 26;13(6):2049–2058. doi: 10.1021/acs.molpharmaceut.6b00187

Figure 3.

Figure 3

Effect of endocytosis inhibitors on internalization of YC6PL in A549 cells. Cells were pretreated with HCQ, chlorpromazine, amantadine, genistein, nocodazole, and MβCD for 2 h before incubation with YC6PL for 30 min. Percentage intensity of green fluorescence (n = 12) compared to YC6PL treated cells was plotted. Pretreatment of the cells with caveolae dependent uptake inhibitors, genistein and MβCD, prior to incubation with the YC6PL showed most prominent reduction in fluorescence intensity compared to other types of endocytosis blockers.