IL-33 increases MDSCs and prevents cyclosporine A-induced hypertension and vascular and renal injury. A, Systolic blood pressure was measured daily prior to ip injections of DMSO-saline vehicle (Control), cyclosporine A (CsA), and IL-33. Aortic relaxation responses to acetylcholine (B) and sodium nitroprusside (C) were measured after contraction with phenylephrine on day 8 following euthanization. D, Aortic and renal fibronectin levels were measured by immunoblotting and normalized to the loading control actin. E, Representative glomeruli and glomerular injury index score for each group. F, Splenic MDSC levels as determined by flow cytometry. Results are expressed as mean + SEM. *P<0.05 vs. Con and n=6–8 mice in each group. There were no significant group × treatment interactions as determined by 2-way ANOVA or repeated measures 2-way ANOVA.