Figure 3.

Effects of acute capsazepine (CPZ) treatment at a dose of 10 mg/kg on occurrence of acoustically evoked seizures in GEPR‐3s. The anticonvulsant effects of CPZ were evaluated on the prevalence and severity of seizures in both female and male GEPR‐3s at different posttreatment time points of 0.5, 1, 2, and 24 h. CPZ at a dose of 10 mg/kg (ip) markedly suppressed or reduced the incidence of WRS in female (n = 8, panel A) and male (n = 8, panel B) GEPR‐3s, respectively. CPZ also suppressed or reduced the prevalence of clonus in female (panel C) and male (panel D) GEPR‐3s, respectively. CPZ delayed the onset of seizure onset in either female (panel E) or male (panel F) GEPR‐3s. Note that seizures were completely suppressed 2‐hour posttreatment, and only one animal exhibited seizure 24‐hour following CPZ treatment. Time course of the effects of CPZ showed a long‐lasting seizure suppression in female GEPR‐3s by the first half‐hour posttreatment (panel G). In male GEPR‐3, however, a long‐lasting attenuation of seizure severity was observed by the 2nd hour posttreatment time point (panel H). The prevalence of WRS and clonus, seizure latency, and seizure severity was analyzed as described in Figure 1. Opened and filled bar graphs represent controls (pre‐CPZ) and CPZ‐treated GEPR‐3s, respectively. *P < 0.05, **P < 0.01, ***P < 0.001