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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Proteins. 2017 Nov 11;86(1):88–97. doi: 10.1002/prot.25411

Fig. 1. TAP-MS of His-FLAG DEK identified 167 potential DEK interacting partners.

Fig. 1

(A) Western blot analysis identified enrichment of HF-tagged DEK by tandem FLAG and Talon affinity purification. MIEG lysates are indicated by (−), HF-DEK by (+). (B) Silver stain of (A) indicated enrichment of HF-DEK and other proteins compared to MIEG control. (C) Lanes from MIEG and HF-DEK samples were sent to the Taplin Mass Spectrometry Facility for analysis. A total of 163 potential DEK interacting factors were identified by TAP-MS. Of these hits, 123 were unique to the HF-DEK sample and are listed below the Venn diagram. The number of peptides and scores for each hit are available in Table S-I. (D) DDX21 and IMPDH2 were confirmed to interact with DEK by Western blot analysis.