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. Author manuscript; available in PMC: 2018 Dec 13.
Published in final edited form as: Cell Host Microbe. 2017 Nov 30;22(6):757–765.e3. doi: 10.1016/j.chom.2017.10.020

Figure 5. Direct actions of antibiotic treatment on immune cells inhibit phagocytic killing.

Figure 5

(A) Changes in macrophage oxygen consumption rate in control (CTL) and cells pre-treated for 3 h with cipro, following electron transport chain uncoupling by 2 μM oligomycin, 1 μM FCCP and 0.5 μM rotenone + antimycin A.

(B) Changes in respiratory capacity following cipro pre-treatment.

(C) Pathogen engulfment by control (CTL) or macrophages treated with 20 μg/mL cipro (ABX), 10 mM amp (amp) or their combination (ABX + amp).

(D) Pathogen survival in CTL, ABX, amp or ABX + amp macrophages.

(E) Phagocytic killing by CTL, ABX, amp or ABX + amp macrophages.

Data are represented as mean ± SEM from n ≥ 3 independent biological replicates. Significance reported as FDR-corrected p-values within the indicated comparisons: *: p ≤ 0.05, **: p ≤ 0.01, ****: p ≤ 0.0001.