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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Hypertension. 2017 Nov 20;71(1):160–167. doi: 10.1161/HYPERTENSIONAHA.117.10268

Figure 5.

Figure 5

Signal transduction changes in kidneys after the induction of BP1. A, Gene expression data were subjected to an analysis for Upstream Regulators using the Ingenuity platform (see Supplemental Methods). z-scores and p-values (−log) are shown. Relevant data points are labeled (red symbols). A complete list (grey symbols) is provided in Table S1. B, Hallmark pathways from a Gene Set Enrichment Analysis of the gene expression data (see Supplemental Methods). NES, normalized enrichment score; q, false discovery adjusted p-value. A complete list is provided in Table S2. C, Detection of phospho-MKK4 or phospho-p38 or phospho-JNK by immunohistochemistry of kidneys from BP1 OFF and ON mice (total n=3). Size bar: 50 μm. Note: Positive immunoreactivity results in dark brown staining. D, Phospho-p38 Western blot analysis of kidney extracts from BP1 OFF and ON animals after immunoprecipitation for phospho-p38. Total p38 protein is shown for comparison.