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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Hypertension. 2017 Nov 6;71(1):125–133. doi: 10.1161/HYPERTENSIONAHA.117.10205

Figure 5.

Figure 5

The effects of CALN1 transduction by lentiviral technique in HAC15 cells. (A) Fluo4-AM fluorescence, index of intracellular Ca2+, was compared between control and CALN1 cells. *P<0.05 vs. control (n=3). (B) R-CEPIAer was applied to compare intra-ER Ca2+ between control and CALN1 cells. *P<0.05 vs. control (n=3). (C) Aldosterone levels in media in HAC15 cells were compared between control and CALN1 cells. *P<0.05 vs. control (n=3). (D) CYP11B2 mRNA levels in control and CALN1 cells are shown. *P<0.05 vs. control (n=3). (E) The effects of TMB-8, an inhibitor of sarcoendoplasmic reticulum calcium transport ATPase (SERCA), on aldosterone production in HAC15 cells with angiotensin II (A-II) stimulation or CALN1 over-expression. *P<0.05 vs. HAC15 cells with A-II stimulation and without TMB-8 (n=3). **P<0.05 vs. HAC15 cells with CALN1 over-expression and without TMB-8 (n=3). (F) The analysis of 2-APB, an inhibitor of IP3, for aldosterone production in HAC15 cells mediated by A-II or CALN1 transduction. *P<0.05 vs. HAC15 cells with A-II stimulation and without 2-APB (n=3).