Fig. 8.
Effect of vitamin E and overexpression of Msr1 on DC cross-presentation. a, b DCs were generated from HPCs with GM-CSF and Flt3L and exposed to TES. Cells were pre-incubated with Vit. E and after 8 h TES was added. a The number of large LB per cell (CD11c+ CD103+ CD172a− DCs). Typical example of staining with BODIPY and the proportion of DCs with the presence of large LB are shown. Scale bar = 50 µm. b Cross-presentation of long OVA-derived peptide by DCs. *p < 0.05, **p < 0.01, ***p < 0.001. c Typical example of the overexpression of Msr1 in DC after infection with Msr1 lentiviral vector. d Proliferation of OT1 T cells, stimulated with Msr1− or Msr1+ DC, loaded with OVA long or short peptides. DC: T cell ratio 1:40 is shown. Proliferation was measured in triplicates. Mean and SD are shown. Two experiments with the same results were performed. *p < 0.05. e Accumulation of lipids in Msr1+ DC and Msr1− DC treated for 48 h with 100 nM LA or ox-tr-LA an then stained with Bodipy. Two experiments with the same results were performed. f Expression of pMHC and H2Kb in DC treated with 100 μM LA or ox-tr-LA and then loaded with long (cross-presentation) or short (direct presentation) OVA-derived peptide. Three experiments were performed. Mean and SD are shown. *p < 0.05. Bars represent standard deviation (SD). Statistical analysis by unpaired two-tailed Student's t test with significance determined at *p < 0.05, **p < 0.01 and ***p < 0.001