Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 14;7:17556. doi: 10.1038/s41598-017-17752-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Evaluation of the content of microglia, blood-derived macrophages and leukocytes in C6 rat gliomas. (A) Percentages of microglia, blood-derived macrophages and leukocytes in brains of sham-operated and C6 rat glioma bearing animals at day 21^st after implantation. Lower panel shows gating strategy and representative results of FACS analysis. Quadrant gates were drawn on three cell subpopulations based on differences in the surface expression of CD11b and CD45 antigens: microglia (CD11b⁺CD45^low), blood-derived macrophages (CD11b⁺CD45^high) and leukocytes (CD11b^-CD45^high). Leukocytes CD11b^-CD45^high and CD11b⁺CD45^high invading macrophages/monocytes were mostly absent in sham-operated brain samples. (B) Kinetics of accumulation of microglia, peripheral macrophages and leukocytes within the tumor. Changes in cell type content at 8^th, 14^th and 21^st day after implantation were calculated as content of each subpopulations in glioma bearing brains related to its content in sham-operated animals (N = 4–6 per time point). (C) Double staining of Iba1 + and Arginase 1 shows accumulation and the pro-tumorigenic activation of GAMs only in tumor-bearing hemispheres (N = 3–5 rats per group).