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. 2017 Dec 14;7:17556. doi: 10.1038/s41598-017-17752-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of MDSCs and T cell subpopulations in rat C6 gliomas. (A) Representative graphs show the analysis of percentages of T regulatory (FOXP3⁺), T helper (CD4⁺), T cytotoxic (CD8⁺) lymphocytes and MDSCs (GR1⁺ within CD11b⁺ cell population) in tumor-bearing hemispheres at the 21st day after C6 glioma cell implantation. Histograms from different subsets isolated from gliomas (dark lines), sham groups (light lines) and IgG control (dotted lines). (B) Quantification of percentage of specific cells among all counted cells isolated from sham-operated and tumor-bearing hemispheres at the 21st day after C6 glioma cell implantation (N ≥ 6 per group). (C) Quantification of percentage of specific cells among all counted cells isolated from peripheral blood of sham-operated and tumor-bearing animals (N ≥ 6 per group).