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. 2017 Dec 14;8:2120. doi: 10.1038/s41467-017-02214-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Ubiquitously expressed elongation factor 1 alpha promoter transgenic lines. a NL1 line expressing enchanced GFP (eGFP). b NL3 line expressing codon-optimized Cherry (oCherry). c NL20 line expressing codon-optimized nuclear localized H2B::oGFP fusion. Right column—single cells from a macerated animal showing nuclear localization of GFP. FITC—FITC channel; DsRed—DsRed channel; BF—bright-field; Hoechst—DNA staining by Hoechst. Scale bars are 100 μm