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. 2017 Dec 14;8:2120. doi: 10.1038/s41467-017-02214-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Tissue-specific promoter transgenic lines. a NL9 line expressing GFP under the muscle-specific promoter of the MYH6 gene. Zoom in—detailed images of the body wall (top) and stylet (bottom); In situ—whole-mount in situ hybridization expression pattern of MYH6 transcript. b NL22 line expressing GFP under the gut-specific promoter of the APOB gene. Zoom in—detailed images of the gut side (top) and distal tip (bottom); In situ—whole-mount in situ hybridization expression pattern of the APOB transcript. c NL21 line expressing GFP under the testis-specific promoter of the ELAV4 gene. Zoom in—detailed images of the testis (top) and seminal vesicle (bottom); In situ—whole-mount in situ hybridization expression pattern of the ELAV4 transcript. d NL23 line expressing GFP under the ovary-specific promoter of the CABP7 gene. Zoom in—detailed image of the ovary and developing egg; In situ—whole-mount in situ hybridization expression pattern of the CABP7 transcript. e NL24 line expressing in a single construct NeonGreen under the testis-specific promoter of the ELAV4 gene and Scarlet-I under the ovary-specific promoter of the CABP7 gene. Zoom in—detailed images of the testis (top) and ovary (bottom) regions. FITC—FITC channel; DsRed—DsRed channel; BF—bright-field. Scale bars are 100 μm