Table 1.

Efficiency of transgenesis with different reporter constructs and treatments

Reporter	Injected line	Injected DNA	Irradiation treatment	Injected eggs	Positive hatchlings (%)	Germline transmission (%)	Established lines
EFA::eGFP	DV1	PCR	—	269	39 (14.50)	5 (1.86)	NL1
EFA::oGFP	DV1	Plasmid	—	114	28 (24.56)	0	—
EFA::oGFP	DV1	Plasmid	2.5 Gy	42	13 (30.95)	2 (4.76)	—
EFA::oGFP	DV1	Fragment	2.5 Gy	102	4 (3.92)	2 (1.96)	NL7
EFA::oCherry	DV1	Plasmid	2.5 Gy	80	4 (5.00)	1 (1.25)	NL3
EFA::oCherry	DV1	Fragment	2.5 Gy	36	6 (16.67)	3 (8.33)	NL4, NL5, NL6
EFA::H2B::oGFP	DV1	Fragment	2.5 Gy	38	10 (26.32)	2 (5.26)	NL20
ELAV4::oGFP	DV1	Fragment	2.5 Gy	56	29 (51.79)	2 (3.57)	NL21
MYH6::oGFP	DV1	Fragment	2.5 Gy	103	13 (12.62)	1 (0.97)	NL9
APOB::oGFP	DV1	Fragment	2.5 Gy	65	2 (3.08)	1 (1.54)	NL22
CABP7::oGFP	DV1	Plasmid	—	20	2 (10.00)	1 (5.00)	NL23
CABP7::oNeon Green; ELAV4:: oScarlet-I	NL10	Plasmid	—	137	3 (2.19)	2 (1.46)	NL24