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. 2017 Dec 14;7:17600. doi: 10.1038/s41598-017-17928-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Recombinant human (rh)SCARF-1 mediates the adherence of CD4⁺ T lymphocytes in the presence of rhVCAM-1, under conditions of flow. (a) A schematic representation of the immobilised protein flow assay. (b and c) Quantification of CD4⁺ and CD8⁺ T cells adhered to immobilised rhVCAM-1 (10 µg/ml) and VCAM-1 in the presence of rhSCARF-1 (10 µg/ml). (d and e) Quantification of CD4⁺ and CD8⁺ T cells adhered to immobilised rhVCAM-1 and rhSCARF-1 pre-treated with isotype matched control (IMC; 10 µg/ml) or SCARF-1 blocking antibody (10 µg/ml). ** and ****indicate statistical significance where p ≤ 0.01 and p ≤ 0.001, respectively. n = 3 independent experiments with different lymphocyte donors, with 12 fields of view taken from each.