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. 2017 Dec 14;7:17608. doi: 10.1038/s41598-017-17962-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of immature retinal astrocytes (Pax2⁺/GFAP^lo) in Vegfr-2 ECKO mice. Retinas were isolated at P5 from floxed and Vegfr-2 ECKO mice, fixed in 4% PFA, and incubated first with anti-Pax2 and anti-GFAP and then secondary antibodies. Stained retinas were flat-mounted and imaged by confocal microscopy. (A–F) images from areas at approximately 0.7 mm from the ONH. (G–I) GFAP:Pax2 ratio (G), number of Pax2⁺ cells per 0.02 mm² tissue area (H), and IntDen values per Pax2⁺ nucleus (I). n = 6. *p < 0.05; ***p < 0.001. Errors bars are SEM. Scale bars: 50 µm.