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. 2017 Dec 14;7:17599. doi: 10.1038/s41598-017-17779-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-course of early-G1 synchronised Pcf T. brucei. Cells samples were withdrawn hourly, stained with PI and analysed by flow cytometry (n = 50,000). (a) Flow cytometry trace of first cell cycle post-elutriation representing the PI signal (horizontal axis) versus cell count (vertical axis). (b) Bar chart of flow cytometry data for the first cell cycle post elutriation. (c) Bar chart of flow cytometry data for the third cell cycle post-elutriation. (d) Box plot of cell dimensions determined by CASY model TT (n > 1,500). Boxes represent the median and central quartiles, whiskers represent 9 and 91% of data, and * the mode.