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. 2017 Dec 14;7:17599. doi: 10.1038/s41598-017-17779-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry time-course of distinct G1 synchronised Pcf T. brucei. (a) Early-G1 cells (97% G1) eluted at 18 ml/min, (b) Mid-G1 cells (92% G1) eluted at 20 ml/min, (c) Late-G1 cells (82% G1) eluted at 22 ml/min. (d) Direct comparison of the proportion of G1 (left) and 1N1K cells (right) over time for the Early-, Mid- and Late-G1 fractions. Left panel - Flow cytometry of PI stained cells at hourly intervals post elutriation (n = 50,000 per time point). Right panel – Microscopy of DAPI stained cells (n > 200 per time point).