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. 2017 Dec 14;7:17565. doi: 10.1038/s41598-017-17770-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AR-12 and AR-14 long-term treatment cured prion infection in ScMEF cells infected with Me7 prions. (a–d) ScMEFs (Me7) were treated with AR-12 (3 µM) or AR-14 (2 µM). DMSO-treated cells were used as a control. Treatment was continued for five passages (20 days). Then, the treatment was stopped and cells were passaged five times again (20 days). (a,f,k and p) Immunoblots showing the effect of treatment with AR compounds on PrP^Sc throughout the experiment compared to DMSO-treated cells. Immunoblots were developed with anti-PrP mAb 4H11 and re-probed for actin. (b,g,l and q) RT-QuIC analysis for uninfected MEF cells at every passage. Each quadruplicate RT-QuIC reaction was seeded with 2 μl of cell lysate (at dilutions 10⁻¹ to 10⁻⁴). The average increase of Thioflavin-T fluorescence of replicate wells is plotted as a function of time. Y-axis represents relative fluorescent units (RFU) and x-axis time in hours. (c,h,m and r) RT-QuIC analysis for DMSO-treated cells. Passages 1 and 5 (P1 and P5) are shown (c,h). After discontinuation of the treatment, passages 1 and 5 (P1* and P5*) are shown (m,r). (d,i,n and s) RT-QuIC-analysis for cells treated with AR-12. (e,j,o and t) RT-QuIC analysis for cells treated with AR-14.