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. 2017 Dec 14;7:17564. doi: 10.1038/s41598-017-17690-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of HRV (SDNN) in wildtye and NPR-C^−/− mice after correcting for heart rate. (A) Plot of SDNN in NPR-C^+/+ and NPR-C^−/− mice in different conditions as a function of HR. Data were fit with an exponential function to correct for HR as detailed in the methods. (B) Linear regression analysis of uncorrected SDNN as a function of HR in NPR-C^+/+ and NPR-C^−/− mice (same mice as used in panel A). (C) Linear regression analysis of corrected SDNN as a function of HR in NPR-C^+/+ and NPR-C^−/− mice. Correlation coefficients in panels B and C obtained using Pearson’s correlation. (D) Summary data illustrating corrected SDNN in NPR-C^+/+ and NPR-C^−/− mice. *P < 0.05 vs. NPR-C^+/+ within activity phase by two-way ANOVA with the Holm-Sidak posthoc test; n = 10 NPR-C^+/+ mice and 10 NPR-C^−/− mice.