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. 2017 Dec 14;7:17550. doi: 10.1038/s41598-017-18019-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chemosensation and dye-filling defects in P. pacificus IFT mutants. (a) 1-Octanol avoidance behaviour of RS2333 and the IFT-related mutant alleles generated in this study. For statistical analysis see Suppl. Table S10. (b) Overlay of bright field and fluorescent images showing dye-filling staining of amphid neurons in a RS2333 adult individual. (c) Dye-filling staining of amphid neurons in a RS2333 adult. (d) Dye-filling staining of amphid neurons in an osm-3(tu1090) adult. (e) Dye-filling staining of amphid neurons in a Ppa-klp-20(tu1131) adult. (f) Dye-filling defective staining of amphid neurons in an Ppa-osm-3(tu1090); Ppa-klp-20(tu1131) double mutant.