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. 2017 Dec 14;7:17550. doi: 10.1038/s41598-017-18019-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hyperoxia avoidance behaviours in P. pacificus IFT mutants. (a) Regulation of bordering behaviour by oxygen levels in RS2333, RSB001 and representatives of Ppa-ifta-1, Ppa-che-11, Ppa-dyf-1 and Ppa-xbx-1 mutants. [O₂] shifts every hour from 21% to 10% to 21%. Three replicates were performed for each strain/mutant. Bar-plots arrows represent the standard error of the mean (SEM). For statistical analysis see Suppl. Table S11. An example of the influence of oxygen in the dynamic of clumping/bordering behaviours for tu1072 is shown under the bar-plot. (b) Ω-turn rate response to 21% −>20% −>21% [O₂] shifts on a lawn of E. coli OP50 of RS2333 and all cilia-related mutants generated using CRISPR/Cas9 system in this study. In all graphs, the black line represents the mean Ω-turn and the grey area, the S.E.M. For statistical analysis see Suppl. Table S12.