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. 2017 Dec 14;7:17571. doi: 10.1038/s41598-017-17931-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of isoforms of OAS1 in PBMC and EBV-transformed B cell lines from patients with SS. (A) The schismatic figures show the four transcript variants of OAS1 mRNA. The grey boxes represent exons of OAS1. The SAS SNP rs10774671 determines the splicing of OAS1 mRNA. Four pairs of primers designed specifically detect each isoform of OAS1 are indicated. (B) Quantitative PCR analyses of expressions of isoforms in PBMC from 6 SS patients and in 6 EBV-transformed B cell lines (C) were performed. The isoforms (OAS1_V1 and OAS1_V3) were amplified with a same pair of primers and were resolved on the same gel. The isoforms OAS1_V2 and OAS1_V4 were amplified with two pairs of primers designed specifically for the V2 and V4 and were resolved separately. The GAPDH amplicon from each sample was used as a loading control.