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. 2017 Dec 14;8:2132. doi: 10.1038/s41467-017-02285-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Temporal analysis of fluctuation in p35S:NLS-KikGR and pUBQ10:NLS-KikGR lines. a Confocal laser scanning microscopy (CLSM) images of p35S:NLS-KikGR before (pre) and after conversion (0 h, 3 h and 6 h). b CLSM images of pUBQ10:NLS-KikGR before (pre) and after conversion (0 h, 3 h and 6 h). Scale bar: 50 µm. c Scatter plot of p35S:NLS-KikG expressing cells (n = 103) obtained from one representative leaf. The normalized mean fluorescence intensity of the cells at 3 h is plotted against the normalized mean fluorescence intensity of the cells at 6 h. Data points are shown in grey, overlapping data points appear black. d Scatter plot of pUBQ10:NLS-KikG expressing cells (n = 55) obtained from one representative leaf