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. 2017 Dec 14;7:17589. doi: 10.1038/s41598-017-17421-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localisation of bioreporter bacteria on the leaf surface of A. thaliana. (A and B) Representative confocal laser scanning microscope images (Z-stack mode to construct 3D image) showing yellow fluorescent chloromethane-bioreporter bacteria (green spots) on the surface of a wild type A. thaliana leaf, with autofluorescent chlorophyll (red) from plant cells underneath. (C) Phase contrast image merged to the corresponding fluorescence image shows fluorescent chloromethane-bioreporter bacteria (green spots), at stomata (black arrows), and the intercellular grooves of leaf epidermal cells (white arrows) of an inoculated wild-type plant.