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. 2017 Dec 14;7:17595. doi: 10.1038/s41598-017-18034-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PNPLA7 expression is lower in the WJ MSC from obese donors. (a) The transcriptome array data were validated using PNPLA7-specific qPCR. Mann-Whitney test showed that there is indeed a significant difference between the two groups (****p ≤ 0.0001). The mean ± SD of the gene expression level was 20.6 ± 2.55 in the non-obese group and 4.3 ± 2.16 in the obese group. Analysis based on the Bootstrap model showed that the 95% confidence interval of the incidence rate ratio was 0.18 (0.15 to 0.21). (b) The GeneMANIA Cytoscape plugin PNPLA7 gene function prediction using association approach. (c) Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) predicted protein–protein interactions for PNPLA7. (d) Mann-Whitney test of the Western blot data indicates that the difference between the two groups is also maintained at the protein level (*p ≤ 0.01).