FIG 3.

Universal essential genes. (A) Overlap between genes defined as essential in this study and three other studies (22–24). The indicated overlap is between all essential genes identified in each study, i.e., an amalgam across all cell lines, in order to assess interlibrary reproducibility. (B) Clustergram of essential genes across 10 different cell lines from the CRISPR screens in panel A. (C) Biological processes enriched in UE genes. (D) Number of essential genes shared between cell lines. Experimentally observed essential genes are shown as a histogram. The indicated models to account for shared essential genes were fitted using maximum likelihood. See the text for details. (E) Fraction of essential genes with orthologous nonessential or essential yeast genes as a function of cell line number. (F) Average essentiality rank of each essential gene as a function of cell line number. (G) Fraction and relative enrichment of UE proteins in specific protein-protein interaction network motifs from BioGRID.