FIG 1.

PKA signaling prevented ADR-induced podocyte apoptosis. (A and B) Representative immunoblot and corresponding graph of podocytes treated with ADR (0.25 μg/ml) in the presence or absence of pCPT-cAMP (50 μmol/liter) for the indicated periods. Each bar represents data obtained from five independent experiments. (C and D) Detection of cell apoptosis by annexin V-PI staining. Data were obtained from at least three independent assays. (E) Evaluation of cell viability using the CCK-8 assay. (F) JC-1 staining of podocytes (original magnification, ×200). Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was used as a positive control of mitochondrial membrane potential decrease. *, P < 0.05 compared with the control group; **, P < 0.05 compared with the ADR group. pCPT, pCPT-cAMP.