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. 2017 Dec 13;38(1):e00181-17. doi: 10.1128/MCB.00181-17

FIG 6.

FIG 6

pCPT-cAMP alleviated ADR-induced proteinuria, renal oxidative stress, and loss of podocytes. (A) pCPT-cAMP increased p-CREB expression in mouse glomeruli (original magnification, ×400). (B) Albuminuria was expressed as the albumin-to-creatinine ratio (ACR) in four groups. Data are expressed as means ± SEM. (C) Urine proteins were stained with Coomassie brilliant blue and were resolved by gel electrophoresis. BSA, bovine serum albumin. (D) Immunohistochemical staining was performed to detect WT-1-positive cells in glomeruli (original magnification, ×400). WT-1-positive cells were counted by a single renal pathologist using a blinded method. At least 50 glomeruli per kidney were found. (E) Real-time PCR analysis of the expression of mitochondrial complex mRNAs in isolated glomeruli. (F) Immunofluorescence staining of 3-nitrotyrosine in mouse kidney (original magnification, ×400). Nestin is a marker protein of mature podocytes. *, P < 0.05 compared with the control group; **, P < 0.05 compared with the ADR group.