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. 2017 Dec 14;92(1):e01580-17. doi: 10.1128/JVI.01580-17

TABLE 2.

Susceptibility to NAIs and viral properties caused by the selected NA substitutions in avian N5 influenza viruses

Influenza virus NA amino acid change (N2 numbering) Selection NAIa NA activity (nM) ± SD IC50 ± SDb (fold change)c
Mean infectivity (log10 PFU/ml) ± SD Mean plaque size (mm) ± SD Genetic stabilityd (% read)
Oseltamivir Zanamivir Peramivir
rgN5 WT Not applicable 44,311 ± 405 0.55 ± 0.002 (1.0) 0.17 ± 0.001 (1.0) 0.05 ± 0.001 (1.0) 8.6 ± 0.1 0.79 ± 0.19 Not applicable
rgN5 L91I OS 46,801 ± 913 0.59 ± 0.001 (1.1) 0.20 ± 0.001 (1.1) 0.05 ± 0.001 (1.1) 8.8 ± 0.1 0.71 ± 0.22 NDf
rgN5 E119V OS 13,527 ± 457 31.89 ± 0.009 (58.5) 128.1 ± 0.021 (736.6) 2.88 ± 0.001 (63.9) 8.5 ± 0.1 0.57 ± 0.18g V (100)
rgN5 N147I ZA 36,505 ± 798 0.56 ± 0.001 (1.0) 5.34 ± 0.003 (30.7) 0.15 ± 0.001 (3.3) 8.83 ± 0.2 0.74 ± 0.21 I (89), N(11)
rgN5 K187R OS 48,329 ± 278 0.87 ± 0.001 (1.6) 0.27 ± 0.001 (1.5) 0.05 ± 0.001 (1.0) 8.64 ± 0.1 0.66 ± 0.14 ND
rgN5 Q221H OS 48,749 ± 392 0.58 ± 0.001 (1.1) 0.47 ± 0.001 (2.7) 0.05 ± 0.001 (1.1) 8.65 ± 0.1 0.71 ± 0.18 ND
rgN5 H274Y Inducede 35,023 ± 79 589.80 ± 0.061 (1,081.8) 0.47 ± 0.001 (2.7) 14.04 ± 0.004 (311.7) 7.5 ± 0.1g 0.57 ± 0.10g Y (98), H (2)
rgN5 R292K Induced 15,150 ± 545 525.40 ± 0.042 (963.7) 0.68 ± 0.001 (3.9) 12.78 ± 0.003 (283.7) 8.4 ± 0.1 0.68 ± 0.16 K (83), R (17)
rgN5 L91I+E119V OS 9,820 ± 49 23.58 ± 0.005 (43.3) 96.61 ± 0.016 (555.5) 3.9 ± 0.001 (86.5) 8.34 ± 0.1 0.42 ± 0.13g I (100), V (100)
a

The NAI used in the selection of the corresponding mutations.

b

The values are means obtained from two independent experiments.

c

Fold change relative to the mean IC50s of the rgN5-WT virus.

d

Illumina sequencing was used to analyze the sequence variation.

e

The mutant was introduced for comparison.

f

ND, not determined due to insignificant alteration of NAI susceptibility of the indicated mutant virus.

g

P < 0.05 compared with the corresponding infectivity value or plaque size of WT virus.