FIG 1.

Identification of suppressor mutations of IAV M2Y76A. (A) Schematic depicting the selection of IAV M2Y76A suppressor mutations. MDCK cells overexpressing M2Y76A were infected with rUd-M2Stop virus. After two blind passages, cytopathic effects were observed and viruses were isolated by plaque picking. (B) Low-MOI growth curve of plaque-purified viruses with suppressor mutations for M2Y76A. The dotted line indicates the limit of detection. The asterisk indicates a statistically significant difference compared to rUd-M2Stop-infected cells (P < 0.01 using two-way multiple-comparison ANOVA with Bonferroni posttest). Experiments were done three times, and representative data are shown. Error bars are standard deviations.