FIG 1.

(A) Schematic representation of the full-length HIV-1 LAI2 HSA-mCherry-IRES-Nef reporter virus used in this study and specifically built for the magnetic separation of infected cells. (B) HIV-1 LAI2 HSA-mCherry-IRES-Nef-infected CD4⁺ T cells express Gag and Nef. Western blots of cell lysates of infected CD4⁺ T cells and of uninfected controls assessed 4 days after infection are shown. (C) HIV-1 LAI2 HSA-mCherry-IRES-Nef infected CD4⁺ T cells express HSA and mCherry. At 4 days postinfection, the cells were stained with biotin-conjugated α-mCD24(HSA) antibody and counterstained with FITC-conjugated streptavidin. After formaldehyde fixation, the cells were analyzed by flow cytometry. Dot plots show the frequencies of cells positive for mCherry expression and anti-mCD24 staining. FSC, forward scatter.