TABLE 3.

PrV gB mutants with single mutations in fusion loops in liposome binding and functional assays

^aSingle point mutations introduced in FL1 and FL2 of PrV gB are indicated. The variants containing mutations to alanine are shaded.

^b2L indicates that liposomes were made of 2 lipids: 60% DOPC and 40% CH. ± indicates the presence of a faint band in the liposome fraction (Fig. 7A), indicating weak binding of the protein to liposomes.

^c4L indicates that liposomes were made of 4 lipids: 20% DOPC, 20% DOPE, 20% SM, and 40% CH.

^dThe expression yield and purity of the recombinant ectodomains are indicated relative to those of the WT protein; NC, no change.

^e± indicates marginal complementation of PrV-ΔgB virus (Fig. 9A).

^fNT, not tested.