FIG 1.
Coexpression of P1ΔVP4 and 3CD in Sf9 cells. (A) Schematic diagram of the constructs used in the present study. lef2 and 1629, the upstream and downstream elements for homologous recombination; hr5-Enh, the Autographa californica multiple nucleopolyhedrovirus (AcNPV) homologous region (hr) 5 enhancer; IE1-P, the IE1 immediate-early promoter; p10-P, p10 promoter; IE1-T, IE1 terminator. (B and C) ELISA (B) and Western blotting (C) of Sf9 cell lysates with polyclonal antibodies as indicated. Mock, uninfected Sf9 cells; 3CD, Sf9 cells infected by IEXBac-3CD; P1ΔVP4, Sf9 cells infected by IEXBac-P1ΔVP4; P1ΔVP4 + 3CD, Sf9 cells coinfected by IEXBac-P1ΔVP4 and IEXBac-3CD.