FIG 10.
Anti-VLPΔVP4 sera block EV71 binding to its key receptors. EV71 was incubated with serially diluted anti-VLPΔVP4 or anti-PBS at 37°C for 1 h. The mixtures were then added to heparin-immobilized (A), SCARB2-Fc-immobilized (B), or PSGL-1–Fc-immobilized (C) agarose beads and incubated at 37°C for 2 h. After washing, agarose-bound virus was released by SDS-PAGE sampling buffer and analyzed by Western blotting with anti-VP1 polyclonal antibody. For SCARB2-Fc and PSGL-1–Fc pulldown assays, the SCARB2-Fc and PSGL-1–Fc were also detected using an anti-Fc polyclonal antibody as controls for protein immobilization.