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. 2017 Dec 14;92(1):e01330-17. doi: 10.1128/JVI.01330-17

FIG 5.

FIG 5

Antigenic comparison between VLPΔVP4 and VLPfull. (A) SDS-PAGE analysis of purified VLPΔVP4 and VLPfull. (B) Western blot analysis of purified VLPΔVP4 and VLPfull. M, protein marker. (C) Reactivity of the mature virion and the mixture of 135S A particles and 80S empty capsid with the VP1-N75-specific antiserum in ELISA. (D) Reactivity of VLPΔVP4 and VLPfull with the VP1-N75-specific antiserum in ELISA. (E) Reactivity of VLPΔVP4 and VLPfull with GH loop-specific monoclonal antibody D5 in ELISA. The data are mean OD450 values and SD of triplicate wells. Statistical significances were determined by one-way ANOVA using GraphPad Prism version 5. ns, P ≥ 0.05; ***, P < 0.001.