FIG 8.
Anti-VLPΔVP4 sera inhibit EV71 attachment to susceptible cells. (A and B) Antisera from an individual mouse of each group were pooled and tested for inhibitory effects by preattachment neutralization assay (A) and postattachment neutralization assay (B). The data are means and SD of three triplicate wells. (C and D) Blockade of EV71 attachment to susceptible cells by anti-VLPΔVP4 sera. EV71 was incubated with serially diluted anti-VLPΔVP4 sera as indicated at 37°C for 1 h. Then, the mixtures were added to RD (C) or Vero (D) cells and incubated at 4°C for another 1 h to allow virus attachment. After washing, cell-bound virus was detected by Western blotting with anti-VP1 polyclonal antibody. β-Actin was also detected, serving as the loading control. IB, immunoblotting.