FIG 7.

AmCl sensitivity of rCan viruses. (A) Parental (black circle) and K33S-GPC rCan (red circle) viruses were used to infect Vero cell monolayers grown in the continued presence of the indicated concentrations of AmCl. Inocula were removed after 2 h, and cultures were continued for 24 h. Cell monolayers then were solubilized in buffer containing 1% Triton, and NP production was determined by ELISA. Infection was normalized relative to viral growth in the absence of AmCl. Error bars from 11 replicate ELISA wells are not visible. (B) Sensitivity of rCan viruses following serial passage in medium containing 1 mM AmCl was assessed as described above, albeit at a fixed concentration of 1 mM AmCl. Viruses tested are rCan (black), K33S-GPC rCan (red), p21 K33S-GPC rCan stock obtained after 21 serial passages (green), and K33S/A168T/ΔFP-GPC rCan engineered to match p21 stock (blue). Error bars indicate the standard errors of the means from 2 replicate ELISA wells. Results presented in this figure are representative of at least two independent experiments.