FIG 6.
Detection of PrPSc in neurons, astrocytes, and microglia in a frozen section. The frozen brain section of the Chandler-infected mouse at 60 dpi was subjected to multiple staining of CD11b, GFAP, NeuN, and PrPSc. The images of each cell marker (magenta) and PrPSc (green) are shown. The top image is a merged image of NeuN and PrPSc on the coronal section containing cerebellum and pons (scale, 1 mm). The magnified image of the medial vestibular nucleus (a) and the spinal trigeminal nucleus (b) indicated by white boxes in the top image are shown below (scale, 10 μm). The panel shows merged images of GFAP and PrPSc (far left), CD11b and PrPSc (second from the left), and NeuN and PrPSc (second from the right); a single image of PrPSc is on the far right. The bottom panel shows increased magnifications of the boxed regions in the spinal trigeminal nucleus images. Colocalization of PrPSc and each individual cell marker is shown with arrowheads.