FIG 7.

HSV-1 gM only minimally colocalizes with overexpressed E-Syt proteins. HeLa cells grown on coverslips were transfected for 12 h with plasmids coding for myc E-Syt1 (A), myc E-Syt2 (B), or myc E-Syt3 (C). They were then infected with wild-type HSV-1 for 12 h, fixed, permeabilized, and immunostained with an antibody against myc to detect E-Syt1, E-Syt2, or E-Syt3 (red signals) and with an anti-gM (4C10) antibody to detect infected cells (green signals), while nuclei were stained with Hoechst (blue signals). Scale bar, 10 μm.