TABLE 1.
Proteins identified by proteomicsa
| Accession no. | UniProt ID | GeneID | Description | Mass (kDa) | Total no. of spectra (% coverage) |
|
|---|---|---|---|---|---|---|
| 1st expt | 2nd expt | |||||
| A8K8U1_HUMAN | Q86VP6 | CAND1 | Cullin-associated and neddylation-dissociated 1 | 136.3 | 8 (9) | 12 (11) |
| B3KMV5_HUMAN | Q9BSJ8 | ESYT1 | Extended synaptotagmin 1 | 122.9 | 3 (3) | 3 (3) |
| B4DDH8_HUMAN | Q96N66 | MBOAT7 | Lysophospholipid acyltransferase 7 | 50.6 | 2 (6) | 3 (10) |
| GM_HHV11 | P04288.1 | 2703379 | HSV-1 gM | 51.4 | 26 (27) | 23 (25) |
a
HeLa cells transfected with HA-gM were immunoprecipitated with an HA antibody, resolved on gradient gels, and processed for mass spectrometry. Peptides were analyzed with Mascot and Scaffold using a combined HSV-1 and human in-house trypsin database. The thresholds for detection were 99% for the proteins and 0.1% FDR for the peptides with at least 2 peptides.