Figure 6. Distinct maternal and paternal chromatin compaction in Wapl ^∆ zygotes.

1. Representative images of paternal and maternal nuclei stained with DAPI of Wapl ^fl (n = 15) and Wapl ^∆ (n = 33) zygotes (from two independent experiments using two females per genotype; see also Appendix Fig S5). Top: Wapl ^fl; bottom: Wapl ^∆. Left: cropped z‐slices from the middle section of the nucleus in fire lookup table (Image J). Middle: cropped z‐slices of nuclei separated by 3 μm. Right: maximum‐intensity projection (MIP) of zygotes. Settings were adjusted for z‐slices and MIP individually but in the same manner for Wapl ^fl and Wapl ^∆ zygotes. Images were adjusted in brightness/contrast in the individual imaging channels using ImageJ. Scale bars: 10 μm.
2. MIP of zygotes seen in (A) with blue ramp lookup table to visualize difference in maternal and paternal vermicelli formation around prenucleolar bodies. Arrow indicates additional DAPI‐intense structures in maternal zygotic nuclei. Images were adjusted in brightness/contrast in the individual imaging channels using ImageJ. Scale bars: 10 μm.
3. Coefficient of variation of DAPI intensity for nuclei of Wapl ^fl (n = 15) and Wapl ^∆ (n = 21) zygotes (P‐value = 1.88 × 10⁻⁷, Mann‐Whitney U‐test).
4. Boxplots showing gray‐level co‐occurrence matrix (GLCM) contrast (local variation of intensity) in paternal (gray) and maternal (white) nuclei in Wapl ^fl (n = 15) and Wapl ^∆ (n = 13) zygotes with increasing window sizes. Horizontal lines of the boxplots represent the medians, box limits show the first and third quartiles, whiskers extend by 1.5 * interquartile range from the limits of the box. Two outliers (maternal Wapl ^∆ window 8) with values 3,242.7 and 4,037.4 are not shown.
5. Boxplots showing size of detected bright objects (voxels) inside paternal (gray) and maternal (white) nuclei in Wapl ^fl (n = 15) and Wapl ^∆ (n = 21) zygotes; note the log scale on y‐axis.

Source data are available online for this figure.